Cell concentration, viability, and cell size and aggregation measurement
A sample containing cells in suspension is mixing with Trypan blue dye, then drawn into the Countstar Chamber Slide analyzed by Countstar® Automated Cell Counter. Basis on classic trypan blue staining, integrating advanced optical imaging technology and intelligent image recognition technology. Countstar® Automated Cell Counter not only provide the cell concentration and viability, but also provide the average diameter and average circularity, and show the diameter range and aggregation of cells in histograms etc.
Table of Content
Aggregated Cell Analysis
A. Image of Cell sample;
B. Image of Cell Sample with identification mark by Countstar BioTech software. (Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell).
C. Aggregated Histogram
Some primary cells or subculture cells are prone to aggregate when poor culture state or excessive digestion, thus causing great difficulty in the counting of cells. With the Aggregation Calibration Function, Countstar® can realize a stimulation calculation of aggregations to ensure accurate cell counting and obtain the aggregation rate and the aggregation histogram, thus providing basis for experimenters to judge the state of cells.
Monitoring of Cell Growing
Cell growth curve is a common method for measuring the absolute growth of cell number, an important indicator to determine cell concentration and one of the basic parameters for the culture of the basic biological properties of cells. In order to accurately describe the dynamic change in the number of cells throughout the whole process, the typical growth curve can be divided into 4 parts: incubation period with slow growth; exponential growth phase with large slope, plateau phase and decline period. The cell growth curve can be obtained by plotting the number of living cells (10’000/mL) against the culture time (h or d).
Measuring Cell Concentration and Viability
Countstar® is applicable to cells with diameter between 5-180um, like mammalian cell, insect cell, and some planktons.
Cell Size Measurement
A. Images of CHO cells suspension stained by trypan blue before and after the plasmid transfection.
B. Comparison of CHO cell size histogram before and after the plasmid transfection.
The change of cell size is a key feature and is commonly measured in cell research. Normally it will be measured in these experiments: cell transfection, drug test and cell activation assays. Countstar® provides statistical morphology data, such as the sizes of cells, within 20s.
Countstar® automated cell counter can give the morphological data of cells, including circularity and diameter histograms.
A sample containing cells in suspension is mixing with Trypan blue dye, then drawn into the Countstar Chamber Slide analyzed by Countstar® Automated Cell Counter. Basis on classic trypan blue staining, integrating advanced optical imaging technology and intelligent image recognition technology. Countstar® Automated Cell Counter not only provide the cell concentration and viability, but also provide the average diameter and average circularity, and show the diameter range and aggregation of cells in histograms etc.
- Aggregated Cell Analysis
- Measuring Cell Concentration and Viability
- Cell Size Measurement
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| Figure 3 Counting of aggregated cells. |
B. Image of Cell Sample with identification mark by Countstar BioTech software. (Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell).
C. Aggregated Histogram
Some primary cells or subculture cells are prone to aggregate when poor culture state or excessive digestion, thus causing great difficulty in the counting of cells. With the Aggregation Calibration Function, Countstar® can realize a stimulation calculation of aggregations to ensure accurate cell counting and obtain the aggregation rate and the aggregation histogram, thus providing basis for experimenters to judge the state of cells.
Monitoring of Cell Growing
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| Figure 4 Cell Grow Curve. |
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| Figure 1 Images were captured by Countstar BioTech as cells (Vero, 3T3, 549, B16, CHO, Hela, SF9, and MDCK) in suspension was stained by Trypan Blue respectively. |
Countstar® is applicable to cells with diameter between 5-180um, like mammalian cell, insect cell, and some planktons.
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| Figure 2 Cell Size Measurement of CHO cells before and after the plasmid transfection. |
B. Comparison of CHO cell size histogram before and after the plasmid transfection.
The change of cell size is a key feature and is commonly measured in cell research. Normally it will be measured in these experiments: cell transfection, drug test and cell activation assays. Countstar® provides statistical morphology data, such as the sizes of cells, within 20s.
Countstar® automated cell counter can give the morphological data of cells, including circularity and diameter histograms.
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