跳至主要内容

Cell Line Analysis

Cell concentration, viability, and cell size and aggregation measurement

A sample containing cells in suspension is mixing with Trypan blue dye, then drawn into the Countstar Chamber Slide analyzed by Countstar® Automated Cell Counter. Basis on classic trypan blue staining, integrating advanced optical imaging technology and intelligent image recognition technology. Countstar® Automated Cell Counter not only provide the cell concentration and viability, but also provide the average diameter and average circularity, and show the diameter range and aggregation of cells in histograms etc.


Table of Content
  • Aggregated Cell Analysis
  • Measuring Cell Concentration and Viability
  • Cell Size Measurement

Aggregated Cell Analysis
Figure 3 Counting of aggregated cells. 
A. Image of Cell sample;
B. Image of Cell Sample with identification mark by Countstar BioTech software. (Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell).
C. Aggregated Histogram

Some primary cells or subculture cells are prone to aggregate when poor culture state or excessive digestion, thus causing great difficulty in the counting of cells. With the Aggregation Calibration Function, Countstar® can realize a stimulation calculation of aggregations to ensure accurate cell counting and obtain the aggregation rate and the aggregation histogram, thus providing basis for experimenters to judge the state of cells.

Monitoring of Cell Growing
Figure 4 Cell Grow Curve.
Cell growth curve is a common method for measuring the absolute growth of cell number, an important indicator to determine cell concentration and one of the basic parameters for the culture of the basic biological properties of cells. In order to accurately describe the dynamic change in the number of cells throughout the whole process, the typical growth curve can be divided into 4 parts: incubation period with slow growth; exponential growth phase with large slope, plateau phase and decline period. The cell growth curve can be obtained by plotting the number of living cells (10’000/mL) against the culture time (h or d).


Measuring Cell Concentration and Viability
Figure 1 Images were captured by Countstar BioTech as cells (Vero, 3T3, 549, B16, CHO, Hela, SF9, and MDCK) in suspension was stained by Trypan Blue respectively.

Countstar® is applicable to cells with diameter between 5-180um, like mammalian cell, insect cell, and some planktons.


Cell Size Measurement
Figure 2 Cell Size Measurement of CHO cells before and after the plasmid transfection.
A. Images of CHO cells suspension stained by trypan blue before and after the plasmid transfection.
B. Comparison of CHO cell size histogram before and after the plasmid transfection.

The change of cell size is a key feature and is commonly measured in cell research. Normally it will be measured in these experiments: cell transfection, drug test and cell activation assays. Countstar® provides statistical morphology data, such as the sizes of cells, within 20s.

Countstar® automated cell counter can give the morphological data of cells, including circularity and diameter histograms.
标签：

评论

发表评论

此博客中的热门博文

Countstar® Rigel S3 Fluorescence Cell Analyzer

Countstar® Rigel S3 Fluorescence Cell Analyzer CAR-T Cell cell mediated Cytotoxicity Studies Phenotypic Charaterization of CAR-T cells Viability and Concentration assay for : 1. Quality control of original blood samples 2. PBMC isolation efficiency tests 3. Cultured status of incubated T-lymphocytes 4. Process Monitoring of modified CAR-T cells 5. Final Product released control. Product Overview The Countstar S3 System combines the three fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity through the use of preconfigured biological applications (BioApps). They are designed to simplify routine cell lab tasks，which leaves you time to focus on the research! Excitation light: 375nm, 480nm, 525nm Emission filter：460nm, 535nm...
发表评论
阅读全文

Countstar® BioLab Automated Cell Counter

Countstar® BioLab Automated Cell Counter Trypan Blue Cell Counting Aggregated Cell Analysis Easy Operation Product Overview Countstar BioLab is a cell counting and analyzing instrument developed on the basis of classic trypan blue staining. Integrating advanced optical imaging technology and intelligent image recognition technology. Countstar® BioLab is applicable to cells with diameter between 5-180μm and the density between 1×104-3×107/ml. Key Benefits Small Benchtop Instrument Simple and Easy Operation Fast testing time – less than 20s Maximum loaded 5 samples in one slide “Fixed Focus”– No manual focus adjustment needed Accurate and high reproducibility result – Intra-assay & Inter-assay CV <5% Maintenance and service free Product feature of  laboratory cell counter Easy and fast Within 20 seconds through three steps with one button Small Sample Volume Sample Volume: 20μL, Accuracy CV in measurements of a single counter is < ...
发表评论
阅读全文

Immune Therapeutic Applications

Cell therapy is undoubtedly a new hope to lead the future of biomedicine, but the application of human cells in medicine is not a new concept. In the past few decades, cell therapy has made great progress, and cell therapy itself is no longer a simple collection of cells and infused back. Cells are now often required to be bioengineered, such as CAR-T cell therapy. We aim to provide you with standardized, GMP level equipment for cell quality control. The Countstar product has been accepted by many companies leading the cell therapy, we can help our customer to build a stable, reliable cell concentration, viability monitor system. Table of Content Challenge in Cell count and viability Dual Fluorescence viability counting by Countstar Rigel S2 Determination of T/NK Cell Mediated Cytotoxicity Consistent Cell Counting and Global Data Management Challenge in Cell count and viability During all steps of clinical CAR-T cell manufacturing, the viability and cell count ha...
发表评论
阅读全文