Dual Fluorescence Method Analyzing Blood and Primary Cells
Blood and freshly isolated primary cells or cultured cells may contain impurities, several cell types or interfering particles such as cell debris which will make it impossible to analyze the cells of interest.
Countstar FL with dual fluorescence method analysis can exclude cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result.
Table of Content
AO/PI Dual Fluorescence Viability Counting
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Figure 1 Procedure AO/PI Dual Fluorescence Viability Counting in Countstar FL
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Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as red blood cell, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.
WBCs in Whole Blood
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| Figure 2 Whole blood sample image captured by Countstar FL |
Analyzing the leukocytes in whole blood is a routine assay in clinical lab or blood bank. The concentration and viability of the leukocytes are the vital index as quality control of blood storage. Countstar FL with AO/PI method can accurate distinguish the live and dead state of cells, and also can exclude the interference of red blood cell.
Counting and Viability of PBMC
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| Figure 3 Bright Field and Fluorescence images of the PBMC Captured by Countstar FL |
AOPI Dual-fluoresces counting is the assay type used for detecting cell concentration and viability. As a result, nucleated cells with intact membranes stain fluorescent green and are counted as live, whereas nucleated cells with compromised membranes only stain fluorescent red and are counted as dead when using the Countstar® FL system. Non-nucleated material such as red blood cells, platelets and debris do not fluoresce and are ignored by the Countstar® FL software.
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