跳至主要内容

Blood and Primary Cells

Dual Fluorescence Method Analyzing Blood and Primary Cells

Blood and freshly isolated primary cells or cultured cells may contain impurities, several cell types or interfering particles such as cell debris which will make it impossible to analyze the cells of interest. Countstar FL with dual fluorescence method analysis can exclude cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result.


Table of Content

AO/PI Dual Fluorescence Viability Counting
Figure 1 Procedure AO/PI Dual Fluorescence Viability Counting in Countstar FL

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as red blood cell, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.


WBCs in Whole Blood
Figure 2 Whole blood sample image captured by Countstar FL

Analyzing the leukocytes in whole blood is a routine assay in clinical lab or blood bank. The concentration and viability of the leukocytes are the vital index as quality control of blood storage. Countstar FL with AO/PI method can accurate distinguish the live and dead state of cells, and also can exclude the interference of red blood cell.


Counting and Viability of PBMC
Figure 3 Bright Field and Fluorescence images of the PBMC Captured by Countstar FL

AOPI Dual-fluoresces counting is the assay type used for detecting cell concentration and viability. As a result, nucleated cells with intact membranes stain fluorescent green and are counted as live, whereas nucleated cells with compromised membranes only stain fluorescent red and are counted as dead when using the Countstar® FL system. Non-nucleated material such as red blood cells, platelets and debris do not fluoresce and are ignored by the Countstar® FL software.

标签：

评论

发表评论

此博客中的热门博文

Countstar® Altair Advanced Cell Analyzer for Industry

Countstar® Altair Advanced Cell Analyzer for Industry Product Overview The Countstar ® Altair is designed based on the classic Trypan Blue exclusion principle, integrating the advanced “fix focus” optical imaging bench, the most advanced cell recognition technologies, and software algorithms. Enable to get the information of cell concentration, viability, aggregation rate, roundness, and diameter distribution by one run. To meet the cGMP regulation, Countstar® Atair software, equipped with a powerful data management function, offers a 4-level user access control feature, e-signatures, and log files that is in compliance with the FDA 21 CFR Part 11 regulations. Key Benefits of Countstar  Full Automated Cell Counter All in one design, integrated Computer/touch screen Automatic Detection 5 Samples in one single test sequence, 2.5 min /5 sample. Unrivaled "Fixed focus" patent, no manual focusing. Powerful and user-friendly Data Management Security User Login and ...
发表评论
阅读全文

Countstar® Rigel S6 Advanced Image Cytometer

Countstar® Rigel S6 Advanced Image Cytometer PBMC cell count and Primary Cell Count Cell Apoptosis Cell Cycle Surface Marker Analysis GFP Transfection Efficiency Optional FCS Express Software Product Overview Countstar ® Rigel S6 is an automated image-based cell analysis platform with full-spectrum fluorescence Microscope, FCS Express software, analysis and reporting of both data and image. Countstar® Rigel S6 equipped with 4 fluorescence channel, allow for 13 different fluorescent analysis combinations, support any dye of different wavelength. Excitation light: 480nm, 525nm, 375nm, 620nm Emission filter：460nm, 535nm, 580nm, 600LP, 665LP Key Benefits of  Fluroescence Cell Analyzer All in one design- Integrated Computer/Touch screen Automatic Detection 5 Samples in one single test sequence. Open platform with maximum 4 fluorescence Channel User Friendly Cell Analysis Protocols Unrivaled "Fixed focus" patent, no manual focusing. High Stability an...
发表评论
阅读全文

Countstar® BioLab Automated Cell Counter

Countstar® BioLab Automated Cell Counter Trypan Blue Cell Counting Aggregated Cell Analysis Easy Operation Product Overview Countstar BioLab is a cell counting and analyzing instrument developed on the basis of classic trypan blue staining. Integrating advanced optical imaging technology and intelligent image recognition technology. Countstar® BioLab is applicable to cells with diameter between 5-180μm and the density between 1×104-3×107/ml. Key Benefits Small Benchtop Instrument Simple and Easy Operation Fast testing time – less than 20s Maximum loaded 5 samples in one slide “Fixed Focus”– No manual focus adjustment needed Accurate and high reproducibility result – Intra-assay & Inter-assay CV <5% Maintenance and service free Product feature of  laboratory cell counter Easy and fast Within 20 seconds through three steps with one button Small Sample Volume Sample Volume: 20μL, Accuracy CV in measurements of a single counter is < ...
发表评论
阅读全文