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Yeast Cell Analysis

Precise analysis of Yeast and Monitoring of Cell Growth Yeast is a type of single-celled fungus with many attractive features that are widely used in the brewing industry, commercial production, environmental protection, and scientific research. Yeast has been commonly used in brewing and bread baking from a very long time ago, and many yeasts are used to produce a variety of feeds and industrial nutrients such as Single Cell Protein (SCP). Table of Content Key Benefits of Countstar BioFerm Counting of Yeast Measurement of Diameter Key Benefits of Countstar BioFerm 1. Fast and easy operation, 20s for each sample 2. Dilution Free( 5x104 - 3x107 cells/ml ) 3. Safe handling and disposal of samples with traditional stains such as Methylene Blue 4. Data easily comparable with a hemocytometer 5. Unique “Fixed Focus” image analysis provides reproducible data 6. Low cost and waste per test using disposable, each chamber slide with 5 chambers 7. Maintenance free Yeas...
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Algae Analysis

Precise analysis for different shape of Algae Based on the research of algae, the technology of directional algae bioremediation is purposefully used in the production of health food, medicine and feed, and improvement of water environment. It plays an important role in promoting algae breeding, improving human health and protecting water environment. Countstar ® BioMarine can automatically calculate the concentration, major axis length and minor axis length of algae and generate the algae growth curve, reflecting the growth of the algae. Table of Content Counting of different shape of Algae Screening Target Algae Biomass of Algae Counting of different shape of Algae Figure 1 Counting of different shape of Algae The shapes of algae, such as circular, crescent, filamentous and fusiform, may differ in thousands of ways. The measurement parameters preset in Countstar® BioMarine for different shapes of algae are applicable to most varieties. As for some speci...
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Cancer Cell Research

Applications of Countstarin cancer cell research The Countstar system combines the image cytometer and cell counter into a single bench-top instrument. This application-driven, compact, and automated cell imaging system provides an all-in-one solution for cancer cell research, including cell counting, viability (AO/PI, trypan blue), apoptosis (Annexin V-FITC/PI), cell cycle (PI), and GFP/RFP transfection. Abstract Cancer is one of the main causes of death worldwide, and the development of new cancer treatment methods is of great significance. Cancer cell is the basic research object of cancer, various information needs to be evaluated from the cancer cell. This research area needs rapid, reliable, simple and detailed cell analysis. Countstar system provides a simple solution platform for cancer cell analysis. Table of Content Study Cancer Cell Apoptosis by Countstar Rigel Cell Cycle Analysis of Cancer Cell Viability and GFP Transfection Determination in Cell Viability an...
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Cell Line Analysis

Cell concentration, viability, and cell size and aggregation measurement A sample containing cells in suspension is mixing with Trypan blue dye, then drawn into the Countstar Chamber Slide analyzed by Countstar® Automated Cell Counter. Basis on classic trypan blue staining, integrating advanced optical imaging technology and intelligent image recognition technology. Countstar® Automated Cell Counter not only provide the cell concentration and viability, but also provide the average diameter and average circularity, and show the diameter range and aggregation of cells in histograms etc. Table of Content Aggregated Cell Analysis Measuring Cell Concentration and Viability Cell Size Measurement Aggregated Cell Analysis Figure 3 Counting of aggregated cells.  A. Image of Cell sample; B. Image of Cell Sample with identification mark by Countstar BioTech software. (Green Circle: Live cell, Yellow Circle: Dead cell, Red Circle: Aggregated Cell). C. Aggregated Histogr...
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Blood and Primary Cells

Dual Fluorescence Method Analyzing Blood and Primary Cells Blood and freshly isolated primary cells or cultured cells may contain impurities, several cell types or interfering particles such as cell debris which will make it impossible to analyze the cells of interest. Countstar FL with dual fluorescence method analysis can exclude cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. Table of Content AO/PI Dual Fluorescence Viability Counting WBCs in Whole Blood Counting and Viability of PBMC AO/PI Dual Fluorescence Viability Counting Figure 1 Procedure AO/PI Dual Fluorescence Viability Counting in Countstar FL Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as red blood cell, giving a highly accurate result. In conclusion, the Countstar sy...
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Application in Bioprocessing

Mammalian cells are widely used in Biopharmaceuticals, such as antibodies, vaccine, peptides and secondary metabolites are produced by bioprocessing with mammalian cells. During the whole process from antibody R&D to production, there are many steps need to carry out cell based assay to evaluate the process or quality control. Such as the total cell concentration and viability will define the status of the cell culture. As well as the cell transfection, antibody affinity determine at cell level. Countstar instruments are the image based cytometry, can help monitor from R&D to production processes and ensure reproducibility and consistency. Table of Content Cell Count and Viability by Trypan Blue Viability and GFP Transfection Determination in Cells Affinity of antibody detection on Countstar Rigel Countstar is GMP-ready for 21 CFR Part 11 Cell Count and Viability by Trypan Blue Monitoring and analyzing cell culture with state-of-the-art solutions. Reliable and ...
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Stem Cell Therapy

Determination of viability, morphology and phenotype for stem cell therapy Mesenchymal stem cells are a subset of pluripotent stem cells which can be isolated from the mesoderm. With their self-replication renewal and multi-direction differentiation characteristics, they possess a high potential for various therapies in medicine. Mesenchymal stem cells have a unique immune phenotype and immune regulation ability. Therefore, mesenchymal stem cells are already widely used in stem cell transplantations, tissue engineering and organ transplantation. And Beyond these applications, they are used as an ideal tool in tissue engineering as seeder cells in a series of basic and clinical research experiments. stem cell analysis The Countstar FL can monitor the concentration, viability, apoptosis analysis and phenotype characteristics (and their changes) during the production and differentiation of these stem cells. The Countstar FL has also the advantage in obtaining additional morphologic...
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